I know that many of you have been losing sleep over the questionable quality of my first PCR and gel. Well, the mystery has been solved! My lab partner made the gel with DI water instead of with TAE/agarose solution. Not only is that an easily fixable problem for the future… it’s something that, for once, is completely not my fault! Woo hoo! I have another PCR running right now, I’ll run the gel in the morning, and I’ll post the results ASAP.
~WhySharksMatter
Discovery Institute water? No wonder it was useless.